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First, the structures reveal that the auxiliary cluster is fully protein ligated both in the presence and in the absence of DOIA, clearly ruling out that the possibility that the cluster coordinates substrate and is involved in its deprotonation; the DOIA substrate is instead bound in a hydrophilic pocket located between the radical SAM and auxiliary FeS clusters.
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CML may be the first disease where we achieve fully protein-based diagnostics and therapy response monitoring.
Protein #1, a sulfite reductase (dissimilatory subunit), is fully soluble; protein #2, a translation initiation factor, is 70% soluble; protein #3, a 3-hexulose 6-phosphate synthase, is 50% soluble; protein #4, a polysulfide reductase subunit is fully insoluble.
Some partially or fully unfolded protein molecules are always present in protein solutions, but mostly refold to their native structure.
Errors were determined using the Levenberg Marquardt R-value for the fit of the Hill equation and two controls were run; fully oxidised protein (in 5 mM GSSG) and fully reduced protein (in 5 mM dithiothreitol (DTT)).
We assumed that detection of a fully glycosylated protein band (band C of about 170 kDa, mature protein) suggested correct processing of ΔF508-CFTR (Cheng et al, 1991).
Proteins are dynamic systems, and understanding dynamics is critical for fully understanding protein function.
Based on a fully automated protein structure homology-modelling program, our data suggest that MamK and MamK-like proteins adopt a fold as in MreB and actin.
Using a fully automated protein structure homology-modelling program, we determined putative models for MamK-like and MamK proteins (Fig. 3B, blue and cyan ribbons respectively).
They were less good at starting from scratch, ie, a fully unfolded protein.
Flexible and fully disordered protein regions that fold upon binding mediate numerous protein protein interactions.
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CEO of Professional Science Editing for Scientists @ prosciediting.com