The predominant isotopomers of succinate, malate, and fumarate in C-glutamine-labeled parasites were fully labeled, indicating that most of the carbon skeletons that enter the TCA cycle via glutamate are not continuously cycled through the TCA reactions, and are presumably exported from the mitochondrion.
SsNMR on fully labeled cell envelope of BL21dm expressing T4SScc.
In this case, the main disadvantage is the limited number of conditions that can be compared simultaneously (usually two), and also the large culture time needed to obtain the fully labeled cell populations that are necessary for quantification.
Incubations were performed with either 8 μM mixed amino acids (AAs) (a mixture of 14 AAs, 99 atm % 15N labeled; Cambridge Isotope Laboratories, Andover, MA, USA), 500 μg l−1 fatty acids (FAs) (99 atm % 13C; Cambridge Isotope Laboratories) or 50 μg l−1 NAs (90 atm % 13C; RNA extract from fully labeled 13C P. stutzeri cultures).
We address the algorithmic challenge by using the now well-known concepts from Active Learning, albeit with a significant departure from how Active Learning has traditionally been presented in the literature: we present a human-operated active learning framework, rather than relying on previously collected fully labeled datasets for simulated experiments.
Differently from classic machine learning algorithms applied to computer vision, which typically employ huge datasets of fully labeled images to perform recognition tasks, DVAs can exploit even a few supervisions per semantic category, by enforcing coherence constraints based on motion estimation.
To validate, in a real and challenging clinical scenario, the proposed bacterial load estimation techniques, we built and publicly released a fully labeled large and representative database of both single and aggregated bacterial colonies extracted from routine clinical laboratory culture plates.
To characterize carbon flux repartitioning during co-assimilation of glycogen and acetate at mid-stage meiosis, a concentrated solution of C-fully labeled acetate ([U-C] acetate) was added to two independent sporulating cultures that were pre-incubated for 7.5 h on 10 g/L unlabeled acetate.
Most of the existing feature-based methods and graph-based methods require sufficiently large amount of labeled examples or a fully-labeled graph for training.
For example, Liu et al. [11] use the notation of supervised transfer learning to denote a fully-labeled source domain and a limited labeled target, while unsupervised transfer learning denotes a mostly labeled source with no target domain labels.
Enabling CC usually improves the performance of predictive models on fully-labeled training networks with large amount of labeled data.
