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The calculations were performed using TZVP and TZVPD-FINE parameterizations and fully dissociated and non-dissociated representations of the H-bond acceptor molecules.
However, although it indicated that Gq is not fully dissociated under these maximal stimulation conditions, our method cannot distinguish between a partial dissociation leaving a fraction of non-dissociated heterotrimers with unaltered high FRET efficiency (25%) and a full conformational change of all heterotrimers characterized by a lower FRET efficiency.
Temperature sensitivity was shown for aqueous solutions of macromers with fully dissociated anhydride groups.
The molecular picture of fully dissociated ions in high pressure NaCl water mixtures that has been put forward by previous authors,[4] therefore, is not correct.
Examples from fully dissociated (DN∗AN), hybrid (DNAN) and symmetric nucleophilic displacement (ANDN) transition states are found in the N-ribosyltransferases.
Upon pressure-jump up to 2 kbar, the matured protofibrils grown over several months become fully dissociated into monomers within a few days.
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Within our dataset, it is difficult to fully dissociate what proportion of the difference in efficiency between pH values of 6 and 8 is attributable to an effect due an unknown difference in the chemistry of both effluent wastewaters tested.
As expected, Rab1 did not fully dissociate from fragmented Golgi in cells microinjected with EspG.
Furthermore, the fibrils of both peptides are stable at pH 7.7, partially dissociate at pH 5.5, and fully dissociate at pH 2.5.
The rates at which tetramers 2 4 appear and tetramers 1 and 5 disappear are identical within experimental error, consistent with TTR fully dissociating to monomeric subunits before reassembling.
While intersegmental transfer serves to bring non-contiguous segments of dsDNA close to each other, 1D sliding during homology search could help RecA reorganize the initial synaptic complex in cis without the need for a RecA filament to fully dissociate and rebind to a nearby homology site located within a short distance (up to few hundred base pairs).
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