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Strep-II-tag was fully cleaved off using TEV-protease in 20 mM Hepes, pH 7.4, 2% glycerol, 0.5 mM DTT.
For crystallization the StrepII-tag was fully cleaved off using TEV-protease in 20 mM HEPES, pH 7.4, 2% glycerol, and 0.5 mM DTT.
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Finally, Pentobra was cleaved off the resin and fully deprotected (cleavage of side-chain protecting groups as well as Boc groups on tobramycin) by treatment with a trifluoroacetic acid mixture containing scavengers.
Similar results were reported in the study on L. rubellus earthworm (Cho et al. 2004) in which a 44 residues were cleaved off from a 283-residue-zymogen to release the fully active proteolytic enzyme.
This amyloid beta is a piece that gets cleaved off and secreted into the extracellular space.
The poly-ubiquitin signal itself is cleaved off and recycled, allowing faster substrate degradation.
Upon illumination the integrin ligand cRGD is cleaved off and cells can no longer adhere on the PEG coating.
Upon refolding, the autoprotease became active and cleaved off its fusion partner, forming an authentic N-terminus.
The fluorophore group was also effectively cleaved off from the labeled products to accommodate easier MS based analysis.
The monolith was then crosslinked by using a low-toxic polymer that could be cleaved off from the monolith hydrolytically.
Low-density lipoprotein particles are taken up by cells and delivered to the lysosome where their cholesterol esters are cleaved off by acid lipase.
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