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At a further prolonging of DNA probe immobilisation time, no noticeable change in the DNA biosensor response was perceived as the binding sites of immobilised AcMPs have fully bound with DNA probes.
As shown in Figure 6, lane 1, naked plasmid DNA moved in the electric field, lanes 3 to 5, no uncomplexed pDNA was observed in the lane when mass ratios of Arg-Eu-HA to pEGFP-N1 plasmid are 30:1, 50 1, and 70 1, respectively, demonstrating DNA have fully bound with nanoparticles.
(B ) Actin filaments fully bound with cofilin over an extended distance.
Although the RepD affinity is much higher with ICRIII present, these measurements were carried out at high RepD concentration; therefore, in either case the DNA should be fully bound with RepD.
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DOI: http://dx.doi.org/10.7554/eLife.02283.027 The apo transport domains in the outward- and inward-facing states are essentially identical and as compact as when they are fully bound, consistent with previous spectroscopic experiments (Focke et al., 2011).
DOI: http://dx.doi.org/10.7554/eLife.02283.019 The structure of the Na+-only bound GltPh-R397A shows HP2 in a conformation overall similar to that observed in the fully bound transporter, but with an opened tip.
Interestingly, Snf2p was recruited to the same region previously occupied by Cyc8 Tup1 in the cyc8 mutant, whilst in the rpd3 hda1 mutant Snf2p potentially co-localised with fully bound Cyc8 Tup1.
Using 3FY-Brd4(1), we detect binding at ligand concentrations as low as 47 μM with chemical shift changes >0.6 ppm when fully bound.
Instead the electropherogram contains zones of fully bound and free aptamer that define an intermediate region reflecting dissociation with increasing run time.
Fully bound GltPh-R397A crystals were obtained in the same crystallization conditions supplemented with 5 mM L-asp.
The lower affinity of this mutant for membranes is likely associated with the reduced efficiency of the transition to the fully bound state.
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