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(B ) Superimposition of the fully bound (grey) and Na+-only bound GltPh-R397A (colors) transport domains.
(A ) Superimposition of the fully bound transport domains (grey) and Tl+-bound GltPhin transport domain in the bound-like conformation (colors), with the averaged anomalous difference Fourier map contoured at 8σ (cyan mesh).
(B ) Side views of thin cross-sections of the closed fully bound (left) and open Na+-only bound (right) intermediate state.
Indeed, the structure of Tl+-bound GltPhin is indistinguishable from the fully bound GltPhin and both Na1 and Na2 sites are occupied by Tl+ ions.
The structure of Na+-bound GltPh-R397A differs significantly from the fully bound GltPh-R397A only at the tip of HP2.
(A ) Surface representations of the protomer in the fully bound intermediate state (PDB code 3V8G) (left), and the modeled Na+-bound intermediate with an open HP2 tip (right) viewed from the extracellular space (top).
Interestingly, because the tip of HP2 forms part of this interface in the fully bound intermediate state of the transporter, opening of the tip in the Na+-only bound form may increase solvent accessibility to the interface.
In an apparent effort to embolden the chancellor, Angela Merkel, he counselled against governments letting themselves "be fully bound by the decisions of their parliaments".For an unelected prime minister, Mr Monti was skating on thin ice.
Many substances are not fully bound by AC.
Results for fully bound cores have also been compared to existing analytical predictions.
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Yes, truth can be hard, and maybe one must be young and not-yet-fully bound up in the system to hear it fully.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com