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Although the size of the hybridization fragment corresponds to the full-size transcript of Helitron-2 (approximately 9 kb), it will be necessary to use other probes complementary to this transposable element to prove that Helitron is really transcribed in D. virilis species.
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Based on the results of this study, we propose that alternative splicing of the clock genes, either by producing truncated isoforms that act as self-regulators or by regulating the abundance of full-size transcripts at the posttranscriptional level, contributes to the precise regulation of the clock function, particularly under fluctuating environmental conditions.
This ssRNA can also be elongated in subsequent cycles since the partial sequence will selectively rehybridize with its anti-sense RNA, leading to a full size' transcript' in multiple ligation cycles.
This results in significant overexpression (∼50%) of a 150-bp minor splice isoform, which produces a protein with an internal deletion of 32 amino acids, and concomitant reduction of the major full size transcripts (2, 5).
In a full-size sedan?
Well, almost full-size.
Full-size, intermediates and compacts.
In F. ananassa, transcript levels of FaMYB10 were detectable but low until fruit were full size.
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Full-length mRNA transcript size was assessed for each progranulin family member by Northern analysis of poly-A enriched mRNA (Micro Poly (A) Purist Small Scale Purification Kit; Ambion) derived from whole adult or 5 dpf animals [ 74].
Full size.
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