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If a result was equivocal, the FISH was repeated on a full tissue section.
The full tissue section was examined and expression score calculated as follows (1 × % cells staining weakly positive)+(2 × % cells staining moderately positive)+(3 × % cells staining strongly positive).
For each case, one full tissue section of invasive adenocarcinoma including the geographic areas tumor center, invasive front and tumor buds were selected for analysis of MACC1 expression by immunohistochemistry. Peritumoral normal mucosa was evaluated for MACC1 expression where available (n = 59).
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The full tissue sections allow large scale histological evaluations with high precision across the complete section.
All normal tissue samples were analysed on full tissue sections.
Full tissue sections were examined to account for rare and focal expression.
Full tissue sections from formalin-fixed, paraffin-embedded surgical resection specimens were cut at 4 μm.
Full tissue sections of benign and tumour human prostate were also used for optimisation of immunohistochemistry and in situ hybridisation.
A good concordance of IHC results has been reported between standard full tissue sections and TMA [ 21].
For diaphragm, full tissue sections were scored for inflammation by a trained veterinary immunologist blinded to drug and genotype.
A potential disadvantage of TMA sections compared with full tissue sections is that donor cores may not be representative of the whole tumor.
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