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Full sections of ca 1 2 mm2 were taken by cutting through the banner using a scalpel to obtain a complete cross section for examination.
The lungs were cut along the long flat frontal plane of the left lobe from side to side to get full sections of the central airways.
To ensure that the absence of staining was not due to regional variation of cellular distribution, the results were validated by repeating IHC analysis on full sections of cancer in selective cases.
Two full sections of tonsil tissue were used as positive and negative controls for each antibody.
Taking into account the focal nature of tumor-associated EMT we used full sections of both primary tumors and metastases to explore SNAI1-associated EMT by immunohistochemistry.
Hypoxia-inducible factor-1 α staining of full sections of DCIS confirmed the presence of hypoxia in cell layers adjacent to the central necrosis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com