With the unprecedented gene density and resolution obtained at a fraction of cost of full-scale sequencing, next-generation sequencing of flow-sorted chromosomes is bound to aid greatly in gene mapping and cloning and the analysis of genome evolution.
High quality sequenced FLICs represent the most useful transcript sequences, and this has led to large scale sequencing of full length insert cDNAs in several non-model species (e.g.[ 21, 23- 26]).
We initiated an analysis of the extent of alternative mRNA editing by large scale sequencing of full-length cDNAs from the bloodstream developmental stage of T. brucei [11], [15].
Together, the above mentioned use of cCDS FLIcs and their source cDNA clones has led to large scale sequencing of full-length inserts in several species [ 18- 23].
Aerobic granulation of activated sludge was successfully achieved in a full-scale sequencing batch reactor (SBR) with 50,000 m d−1 for treating a town's wastewater.
Common techniques and databases, including in silico analyses of expressed sequence tag (EST) databases [ 11, 12], genomic annotation of large transcript sets [ 13- 17], large-scale sequencing of full-length complementary DNAs (cDNAs) [ 18- 20] and tiling arrays [ 21- 24], have been applied to identify NATs.
Sequencing identified the gene as full length ORCTL3 (organic-cation transporter like-3 gene)/SLC22A13, which was originally determined through large-scale sequencing of genomic DNA (20).
Large-scale sequencing of DNA is a new art that the consortium learned by doing it.
From the cacophonous musical lead-in, one could even get a rough sense of the full scale of "Loveland," the climactic sequence in which the characters' past and present finally merge into a nightmarishly surreal Follies show.
Effects of laser parameters and laser shot sequencing on final residual stress distribution are studied by performing full scale simulations of LSP patches constituting a large number of laser shots.
