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The full range of substrates of CYP4V enzymes is not yet known, although human CYP4V2 is a selective omega-hydroxylase of saturated, medium-chain fatty acids [ 86].
Of course there are problems (Table 1): the mitochondria are not directly accessible to the full range of substrates and inhibitors, and the complexity of cytoplasmic metabolism must be considered together with the presence of separate pools of adenine nucleotides, nicotinamide nucleotides and calcium in the cytoplasm and mitochondrial matrix.
Similar(57)
Calpain 3 recognizes a wide range of substrates, including cytoskeletal proteins and myofibrillar proteins [40], [46].
DPP IV acts on a wide range of substrates.
Substrate specificity of the enzyme was tested for a broad range of substrates (4 mg/ml) such as keratin, casein, BSA, gelatin as soluble substrate.
Operation over a wide range of substrate concentrations.
This arrangement has been successfully used in a wide range of substrate types.
However, the full range of cellular substrates of CHIP remains to be explored.
In transgenic plants, CYP105A1 disrupts brassinosteroid homeostasis to give a phenotype, but the full range of potential substrates and the extent to which their levels are altered is not known.
Although both ChiA and ChiB contribute to the chitinolytic activity of L. monocytogenes, the full range of their potential substrates remains to be determined, including the identification of host-related targets.
An important motivation for this work is to comprehensively identify the full range of cofactors that are consumed or produced by potential substrate cycles.
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