Sentence examples for full length target from inspiring English sources

The case of shR-P15 highlights the importance of screening full length target sequences.

Whenever circulating DNA is analyzed, copy numbers derived from a real-time PCR assay with fragmented DNA as a template cannot easily be interpreted as haploid whole genome equivalents because the real-time PCR assay counts only the copies of available full length target.

Suppression PCR limits amplification to DNA molecules representing full length target DNA, while amplification of other types of molecules formed during ligation is suppressed.

Prediction and thermodynamic analysis of secondary structure at a range of temperatures in full length target sequences, as well as in subsequences formed by in silico shearing, revealed the likely presence of stable secondary structures in both full-length target and sheared target mixtures.

(PDF 446 KB) 12284_2012_45_MOESM4_ESM.pdf Additional file 4: Figure S4: Global views of the degradome sequencing signatures along the full-length target genes of rice.

(PDF 58 KB) 12284_2012_45_MOESM2_ESM.pdf Additional file 2: Figure S2: Global views of the degradome sequencing signatures along the full-length target genes of Arabidopsis.

Longer PCR targets did not amplify quantitatively in circulatory DNA, due to limited presence of full-length target sequence in the sample.

However, in both hemizygous and heterozygous full-length target studies the shR-P16 showed strong mutant selectivity with minimal effect against the wild-type.

A positive BAC was sequenced and found to contain the expected full-length target gene, several gene-like regions, and both known and novel repeats.

While analysis of short atxn7 targets revealed that RNAi hairpins incorporating nucleotide mismatches 3' to the central region were most selective for mutant atxn7 silencing, this approach was not predictive of the most efficacious hairpin for full-length target silencing.

Transfections for the hemizygous full-length target fluorescence assays were performed using jetPEI (Polyplus) with an N/P ratio of 5, using a total of 2 µg of DNA in a 1∶1 ratio of target∶shRNA vector.