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Full length message of β1A with either exon 7a or 7b was reassembled in pIRES2-EGFP opened with BglII/SmaI site using the internal HindIII restriction site.
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Various aberrant splice variants could be detected that involved splice donor and acceptor sites of the lentiviral construct, inducing downregulation of Ebf1 full-length message.
Similarly we assumed that the mRNAs for these genes would be trans-spliced, so that PCR with a gene specific reverse primer and a generic trans-spliced leader forward primer using cDNA template would successfully amplify full-length message.
The full-length message of SmINSIG was obtained by RT-PCR.
(B ) The mRNAs encoding consecutive AAA codons result in discrete length toeprint signatures, yielding specific bands corresponding to the full-length message on our toeprints.
The most common mRNA determinants of ribosome pausing on full-length messages are rare codon clusters.
Ribosome stalling can occur at the 3'-ends of non-stop transcripts, or at internal sites within full-length messages.
However, the TERT transcript is alternatively spliced, and only the expression of full-length messages correlates with enzyme activity (Ulaner et al, 1998).
Aside from using it to tell time, users can read full length emails and text messages on the 1.3-inch OLED display, as well as control the music playing on the phone.
Splicing of upstream exons prior to the first coding exon results in messages that differ in their 5' UTRs, and translation of these messages produces full length ERα66.
Northern blot analysis indicated that no full-length ETR1 message was made in the etr1-9 mutant seedlings (Fig. 1B).
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