The engineered soluble enzyme exhibited the same activity as the full length membrane-associated enzyme expressed in yeast.
Mice were immunized by a single i.m. injection of a plasmid expression vector encoding either full length membrane-integral Nogo-A equipped with a signal peptide or two versions of its large N-terminal extramembrane region.
Likewise, the means to rapidly analyze the mass of full-length membrane proteins has led to technical advances to probe dynamics by nonequilibrium techniques.
Thus, we suggest that Sf9 insect cells are highly feasible for the fast and easy production of various budded VLPs that serve as a platform for full-length membrane receptor presentation.
These include the full-length membrane bound receptor, capable of transducing a cellular signal, and a soluble receptor, capable of sequestering ligand or dimerizing with the full-length receptor to prevent signal transduction [33], [34].
The conditioned media had no effect on pH-dependent currents (Fig. 7C), suggesting that the modulatory effect of CHEB42A was not mediated by the secreted peptide, but rather required physical interaction with the full-length, membrane bound protein.
The secreted KL (sKL) and full-length membrane form of klotho have distinct functions.
Ahuja et al. determined the structure of full-length, membrane bound b5 in the presence of CYP2B4.
However, when full-length membrane reconstituted syntaxin is examined, pulse EPR demonstrates that the configuration of the protein is different and predominantly in an open state.
The full-length membrane PTK7 has a pronounced anti-migratory effect in HT1080 cells while its proteolysis reverses this anti-migratory effect and facilitates cell locomotion.
The recent data suggest that proteolysis of the full-length membrane PTK7 involves MT1-MMP, ADAMs and γ-secretase [ 11, 21- 23].
