These data demonstrate that upon infection by a virus that recognizes the viral replication site, a full-length complement of the transgenic transcript is produced.
The presence of full-length complements of viral genomic RNA is a hallmark of RNA virus replication within an infected cell.
Because imbalances in the synthesis of genomic RNAs and their full-length complements are common during RNA virus infections, false priming of the more abundant strand during the RT step of the assay is likely to inhibit accurate quantification of the less abundant strand, when standard unmodified virus-specific primers are used for reverse transcription and qPCR.
As expected, over-expression of full length Rad60 complements the ts and DNA damage sensitivities of rad60-1 (Figure 3B).
The CCM1 ORF lacking PPR2, which is the most canonical motif in the full length Ccm1p, complemented neither B [ 14] nor I Δ ccm1 segregants (J. I. Moreno and M.A. Piva, unpublished work).
MASiVEdb offers, through multiple and often novel ways, a comprehensive, highly curated and detailed report on the full-length Sirevirus complement of each species, while it additionally includes an integrated system for analyzing user-provided sequences against MASiVEdb elements.
A subset of mutants was complemented with the full length gene in trans.
In order to test whether pepper eIF4E alleles can complement the susceptibility to TEV in Arabidopsis, the full length cDNA encoding Ca.eIF4E1 -pvr2 + and Ca.eIF4E1- pvr2 2 were cloned into a binary vector and transformed into eifiso4e rtm1 Arabidopsis plants.
The mutated MCPH1-transcript detected in the breast cancer specimen described above was unable to complement the defective activation of DNA damage response proteins, in contrast to full length MCPH1.
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