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The GFP brightness and cell size under full induction was quantified by fluorescent microscopy.
The flowering response to a single 22-h LD of vernalized plants grown on soil was also strongly reduced: whereas full induction was obtained with 8-week old WT plants, only 13% of 10-week old nia1nia2 plants responded to the LD (Table 3).
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The thiamine-regulatable nmt1 promoter and derivatives, which take >15 hours for full induction, are most commonly used for controlled expression of ectopic genes.
The inability of DevRC to support full induction is attributed, at least in part, to a failure to cooperatively recruit DevR to adjacently-placed secondary binding sites.
Another possible cause of the observed divergence between in vitro and in vivo measurements in full induction is that, in vivo, the number of polymerases and other molecules involved in transcription is limited.
In two cases it is unknown whether the full induction course was received.
The oscillation frequency was 375 kHz, and the full induction power was 5 kW.
However, while PR1 has previously been shown to require CBP60g and SARD1 for full induction, PR2 was not significantly affected.
Furthermore there was no difference compared with cows where more full term induction were used [ 3- 5].
As mentioned, in [ 7] it was reported that, in vitro, when no inducers were present, the mean of intervals between RNA productions events is ~6000 s while for full induction it is ~50 s (~100-fold change).
On the other hand, high expression of the galactokinase before galactose levels are high enough to cause full induction could be deleterious because of the accumulation of galactose-1-phosphate to toxic levels.
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