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A BlastP search of the full fragment sequence confirmed that it was a TSPO-like protein.
Future studies will use PrOF NMR in a full fragment screen.
The pGEM-P2X7 plasmid was constructed by inserting the full fragment of the rat P2X7R sequence (number X95882) into the pGEM-T Easy vector (Promega).
Although the number of segment copies depends on the number of B chromosomes, this correlation is not necessarily direct, as some B chromosomes may lack the full fragment.
The cross-correlation between Watson and Crick reads was used to infer fragment length and reads were extended to the fragment length and recounted for the full fragment using genomeCoverageBed.
Each PCR involved four oligonucleotide primers and resulted in the amplification of a full fragment (control band) and one allele specific fragment (see supplementary materials for further details [Additional file 2: Supplemental Table S12]).
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Macro-XRF maps of the full fragments were used to identify and localize elements both outside and inside of the ink (cf. the supporting information, where all the XRF elemental maps of the five samples are provided and some specific results are commented).
We characterised the full-length MALAT1 by gene sequencing, and the right full fragments with no SNP was cloned into pLV4 expression vector, named pLV4-over/MALAT1.
Overall, the results obtained using the cuPSSM for full fragments were comparable to the SVM results based on overall nucleotide composition when ignoring the position information.
The full length of MALAT1 gene was spliced from above three fragments by series of PCR, following by gene sequencing, and the right full fragments with no SNP (single-nucleotide polymorphism) was cloned into pLV4 expression vector, named pLV4-over/MALAT1.
Fragments of Pdu-pdp1, Pdu-vrille, Pdu-timeless, Pdu- ck1δ/ε sequences and full fragments of Pdu-clock,Pdu-bmal, Pdu-tr-cry and Pdu- 4-6-photolyase sequences were identified based on BAC and EST sequences.
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