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The full field microscope collected fluorescence in the 510 560 nm spectral range (Figure 5).
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Images are collected on Leica wide field microscope.
Images were taken using both an Axiovert200 wide field microscope and a confocal microscope.
The second, full-field microscope gives no spectral information, but global fluorescence intensities for a given spectral range (510 560 nm in this study).
For both scanning and full-field microscopes, samples are usually mounted as a sandwich in between two pure foils of Ultralene (Spex, CertiPrep).
The two combined optic systems have potential applications in micro X-ray diffraction, micro X-ray fluorescence, micro X-ray absorption near edge structure, full field X-ray microscopes and so on.
An arrangement in which a diffuser may be utilised to provide full field speckle illumination in a fluorescence microscope is described.
The second, full field imaging system was a Zeiss Axio observer microscope with a Zeiss Ultrafluar 40x objective.
SEM (Scanning Electron Microscope -based optical Microscope -basedable appropticaldue to their full field, high methodscareon andesirablensitivity.
In addition, this work introduced his specially designed field microscope, which subsequently became known as the Withering botanical microscope.
All examination was performed using a bright field microscope.
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