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After a three-for-one stock split, G.M. is expected to have 1.5 billion common shares outstanding, or 1.8 billion assuming full dilution of the stock.
Rb anti-G544 PC was tested in a full dilution series from 1 25 to 1 1 54,675 using 3-fold serial dilutions.
The percentage of isolates inhibited at each concentration of antifungal agent over the full dilution series is summarized in Table 2.
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Or full anti-dilution protection.
Expired air was collected using a full-face transparent dilution mask (Scott Aviation, Lancaster, NY) connected to the calorimeter by leak-proof tubing (Vacumed, Ventura, CA).
For Syber Green assays, a β-Actin standard curve was first performed using the full cDNA cascade dilutions of each cDNA sample in triplicate and a RotorGene 6000 (Corbett Research, Labgene, Archamps, France).
Two cDNA concentrations (full strength and 1∶2 dilution) were used to calculate ΔCT in order to confirm that the obtained ΔCT values were consistent with the cDNA concentrations.
For enzyme immunoassays, we used 20 μl of sample dilution (for full descriptions of antibodies and validation see Palme and Möstl 1994; Hirschenhauser et al. 1999; Möstl et al. 2001).
For example, for a 0.5 mL vaccine, the following doses may be given at 15 30 minutes intervals [20]:0.05 mL 1 10 dilution;0.05 mL full-strength;0.1 mL full-strength;0.15 mL full-strength;0.2 mL full-strength.
Relative copy numbers were calculated from an 8-point standard curve generated from a 10-fold serial dilution of full-length cDNA constructs as described previously (Leong et al, 2007).
It's also typical to see term sheets that include full-ratchet anti-dilution protection and most-favored-nation clauses.
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