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In this work we have shown that non-invasive E. coli bacteria carrying a functional T3SS are able to secrete and translocate to the cytoplasm of human cells sdAb fragments with full capacity to bind their cognate antigens.
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Furthermore, acylation of each of the Lys or Lys residues alone was necessary and sufficient for conferring CyaA a full capacity to tightly bind its αMβ2 integrin receptor (CD11b/CD18).
Then their capacity to bind dT7 was assayed.
These recombinant domains were assayed for their capacity to bind to human RBC.
Similarly to the β-antigen, CbpA has capacity to bind factor H of complement.
Ang II possesses the capacity to bind to two distinct receptor subtypes, AT1 and AT2 [45].
Importantly, several other members of the bZip family have the capacity to bind the PARRE.
The capacity of TrkAIII to bind α-tubulin adds to its capacity to bind γ-tubulin [ 4].
These results indicate that Nucant binds nucleophosmin independently of its capacity to bind to surface nucleolin.
All of the mutants retained the capacity to bind to HOIL-1L (Supplementary Figure S6A), showing that the mutations did not cause major deficiencies in the folding of the full-length protein.
All members contain a MRG domain which has the capacity to bind multiple transcriptional regulators [ 18].
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