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The treatments were sole barley (125 kg ha−1), sole faba bean (200 kg ha−1) and an additive series of 12.5, 25, 37.5, 50 and 62.5% of the sole seed rate of faba bean mixed with full barley seed rate.
This was done by BLASTN, with the combined set of sequences as query against the full barley chloroplast genome sequence (http://www.ncbi.nlm.nih.gov/nuccore/118430366?report=fasta).
One would expect the number of wheat sequences represented in the full length cDNAs to be lower than that for rice, brachypodium and sorghum simply because the barley cDNA dataset [ 39] does not correspond to the full barley genome.
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The sequences of the two full length barley Type-like MADS-box ESTs were used to interrogate the barley database http://webblast.ipk-gatersleben.de/barley/index.php for detection of genomic sequences.
Finally, the wheat contigs were compared against 23,614 published sequences of full length barley cDNAs [ 39]. 86,631 wheat sequences (67.4%) were represented in the barley full length cDNAs and 19,762 barley sequences (83.7%) were found to have at least one wheat homologue.
The fields are full of barley or traditional British breeds of cattle.
I take a stroll north of the city, which in 1981 was full of barley fields.
And a pilot project in the U.K. recently saw researchers tend a field full of barley using only robots.
Aeneas Tacticus, in a manual of defense strategy, advanced methods for detecting infiltrators, too — sleeper cells sneaking weapons into the city in wagons full of barley.
* full-length barley sequences are given in brackets (total number/new from RNA-seq).
RT-PCR of full-length barley pri-miR159b transcripts demonstrated the presence of both unspliced (+IVS, Inter Vening Sequence) and spliced isoforms (ΔIVS C with almost the same expression level.
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CEO of Professional Science Editing for Scientists @ prosciediting.com