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The full assignment of all NMR peaks is technically impossible.
Full assignment of Raman spectrum of 1 tautomer is given in Table 4.
An extensive and detailed NMR study allowed the full assignment of the chemical shifts of the two diastereomers in the mixture.
From S. incisifolium we isolated the highly unsaturated glycolipid 1-O-palmitoyl-2-O-stearidonoyl-3-O-β-D-galactopyranosylglycerol (4) and we report the first full assignment of its 1H and 13C NMR data.
Herein, we describe the first isolation of 1-O-palmitoyl-2-O-stearidonoyl-3-O-β-D-galactopyranosylglycerol (4) from a brown alga (Sargassum incisifolium) with full assignment of the 1H and 13C NMR signals.
After the full assignment of 1H and 13C NMR data for 2 by HMBC and 1H 1H COSY spectra (Fig. 2), the gross structure of the other parts was the same as 1.
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The full assignments of the aglycon and sugar signals were carried out by HSQC,1H-1H COSY and HMBC experiments.
The full assignments of its 1H and 13C NMR (Table 1) were obtained by 2D NMR (Fig. 2).
Full assignments of all of the observed molecular ions can be found in Table I.
These improved spectral features enabled full assignment (102/102 expected non-proline residues) of the backbone resonances of TGF-β313 using a single 0.4 mM N, C sample and standard triple-resonance experiments implemented on a 700 MHz spectrometer.
For the results of the full assignment, see Supplementary Table S1.
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