The two phase-specific agents we studied, 5-FU (S phase) and vinblastine (M phase), were both ineffective in the multicellular environment of the TSQ.
Given the in vitro synergy between L-OHP and UFT in the HT29 cell xenograft model, and between CPT-11 and 5-FU, some phase I studies have been conducted to define the recommended dose (Louvet et al, 2000; Prince and Hill, 2000; Alonso et al, 2001).
Intracellular iron disruption by chelators leads to cell cycle arrest, particularly in G1 and S phases (Fu and Richardson, 2007; Siriwardana and Seligman, 2013).
For 5-FU analysis, mobile phase of 1% acetonitrile in deionized water was utilized with flow rate 1.0 mL/min, column temperature of 25°C, and UV adsorption detecting at 246 nm [ 25].
The recommended doses of docetaxel and 5-day continuous infusion of 5-FU for a phase II trial are 50 mg m -2) and 500 m -2(-2) per dandevery 3 or 4 weeks.
Several studies have reported encouraging results with single chemotherapy agents or combination treatments (Mallinson et al, 1980; Casper et al, 1994; Palmer et al, 1994; Carmichael et al, 1995; Burris et al, 1997) and gemcitabine has been compared to 5-fluorouracil (5-FU) in a phase III study in patients with advanced pancreatic cancer (Burris et al, 1997).
The GEE analysis demonstrated a significant difference for 5-FU in the G2 phase after 24 h (β=20.2, P<0.001).
LD10 dose of 5-FU resulted in S phase arrest, whilst the LD50 caused G1 arrest, irrespective of incubation periods (Table 2).
So, as the specific chemotherapeutic reagent for killing tumor cells in S phase, 5-Fu was choosed to study the change of sensitivity of breast cancer cells when T3 was present.
This trial is the first to compare the efficacy and tolerance of three different CRT protocols in the treatment of LAPC: based on several efficacy end points (e.g. response rate, PFS, OS), RT-GC and RT-GC+GC were not distinctly superior compared with the reference arm RT-5-FU in this randomised phase II study.
