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An aliquot of RNA samples was spotted on the card or swabs containing field samples were pressed against an FTA card in order to transfer the maximum amount of fluid from swab to the FTA paper.
We demonstrate that the nullomers can be added directly to collection devices (FTA paper) to allow tagging during the process of sample collection.
5 μl samples were spotted onto the FTA paper.
The method is sensitive enough to detect a single organism even when immobilised on FTA paper.
FTA paper, developed by Whatman, is a matrix that binds and lyses cells.
The punches were processed as described above (see "FTA paper - sample stabilization and recovery" section).
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In addition to the highest sensitivity, custom modified application of FTA® paper extraction protocol is the simplest in terms of incorporation into MASSE and displayed little bias in sensitivity with respect to prokaryotic cell type.
The basic premise of purifying DNA using FTA® paper is simple: biological samples are applied to the FTA® paper and air-dried.
Identical PCR products were amplified from both eluted DNA and the FTA® paper (figure 6), although, unlike the avian blood, direct amplification from the DNA in FTA® paper was more efficient than using the eluted DNA.
FTA® paper is a commercial product which consists of filter paper impregnated with a proprietary mix of chemicals.
We describe here the storage of biological samples on FTA® paper (commercially available reagent-loaded papers similar to filing cards).
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