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Like ρ, FST' is not affected by the reduction of Ne due to inbreeding because FST'/ 1- FST') = 1/(4 Ne'. m) in an island model at equilibrium.
Fixation index (FST =1-πiw/πit), where πiw is the average number of different nucleotides between two sequences within populations and πit is the number of different nucleotides in the whole population was obtained.
Following the simplistic [ 53, 54] equation FST ≈ 1/4 Nm + 1), our mean local FST estimates indicate long term Nm = 10.2, and migration rates of 0.17 assuming N e = 60.
To infer population differentiation, we calculated the Fixation index (FST) [ 1, 2].
In a neutral island model, FST/ 1- FST) = 1/(4 Ne. m) at equilibrium, where m is the migration rate among populations.
FST-1 was successfully expressed in E. coli DH5α.
FST-1 activity was identified by the high performance liquid chromatography (HPLC).
Results revealed that FST-1 was identified to 98% similarity with fructosyltransferase mRNA of onion (accession number: AJ006066).
The optimal conditions of FST-1 were analyzed by the dinonylnaphthalene sulfonic acid (DNS) and orthogonal test.
The optimal conditions of FST-1 in catalyzing reaction were the pH 5.0, °C °C and 60% sucrose substrate.
HPLC results indicated that the expressed protein from FST-1 had a good transferring activity for fructose.
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