Several fruit development pathways were analyzed including lignin pathway, sugar and acid pathway, and hormone signaling.
Extensive studies of enzymatic capacity of fruit sugar pathways are needed to improve our understanding of mechanisms involved in sugar metabolism and in the fructose-to-glucose ratio.
In addition, there were no predicted relationships that were not present on the KEGG fruit fly pathway.
A number of proteins related to fruit respiratory pathway and quality (i.e., firmness and soluble solids) exhibited significant changes over the course of fruit maturation and ripening.
Notably, the miR395 family members were identified to target ETHYLENE INSENSITIVE 3 (EIN3), a well-known transcription factor involved in the fruit ripening pathway [ 56].
Based on a combination of results from automated data extraction from the Kyoto Encyclopedia of Genes and Genomes (KEGG; www.genome.jp/kegg/) and the presence of selected flavonoids in our mango extracts [ 13], we also propose here a mango fruit PF pathway, with particular emphasis on the mango signature compounds mangiferin and epicatechin-3 O-gallate [ 32, 33] (Fig. 1).
Hence, we took a deeper look at the miRNAs identified that are involved in several important pear fruit development metabolic pathways, including lignin pathway, sugar and acid pathway, hormone signaling and carbohydrate metabolism activities.
In addition, there are intervention studies that prove a beneficial influence of vegetables and fruit on metabolic pathways that are associated with the risk of CHD.
These results indicate that, in fruit, certain folate pathway genes are developmentally regulated and that certain others are subject to feedforward control by pathway intermediates.
