Sentence examples for frozen vegetative from inspiring English sources
Archegonia-bearing region together with the involucre which shelter archegonia bundle [3 cm × 3 cm in size, Additional file 5: Figure S4] was dissected from the frozen vegetative parts of thalli from around 50 female individuals grown in the natural habitat.
Leave samples (Hermes) were collected at vegetative stage, immediately frozen in liquid nitrogen and stored at -80°C.
Vegetative rosettes were harvested and frozen for RNA isolation or immediately fixed in formaldehyde for isolation of chromatin as described below.
S. viridis vegetative tissues and developing grain were snap frozen in liquid nitrogen immediately after harvest.
Individual receptacles (female and male) were collected, while apical tips (vegetative tissue) were pooled; tissue was fast frozen in liquid nitrogen and stored at -80° before further analysis.
Other loci with minor effects on freezing tolerance at the vegetative stage have been mapped on barley chromosomes 1HL, 4HS and 4HL in the 'Dicktoo' (facultative) × 'Morex' (spring) barley mapping population together with Fr-H1[ 15].
Blades (vegetative material above the ligule) were removed after a 3-week regrowth period, immediately frozen and ground in liquid nitrogen and freeze-dried.
Mixed cultures and vegetative control samples were collected onto 1.2 μm pore-size membrane filters (RAWP04700 Millipore), placed in Trizol™, flash frozen in liquid nitrogen immediately and stored at -80 °C until RNA extractions.
