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Upon arrival at the laboratories, frozen specimens were initially stored at -20°C, refrigerated samples were stored at 4-8°C 4-8°Cfrozen specimens with delandd analysis were stored at -70°C or lower [ 21].
In contrast, the other specimens, especially frozen specimens, resulted in poor IR.
Pollen pellets were carefully removed from frozen specimens and stored in separate 1.5 ml centrifuge tubes.
Frozen specimens were stored at 80°C.
Frozen specimens were transported, if necessary, in dry ice in insulated boxes (Carbagas, Liebefeld, Switzerland).
DNA and RNA were subsequently extracted from the freshly frozen specimens, according to published protocols [ 11].
Formalin-fixed, paraffin-embedded as well as frozen specimens were used.
These frozen specimens were stored at −70°C in a tumour bank until use.
Small samples prepared from freshly collected and frozen specimens were ground with sterile plastic pestles.
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Protein isolated from frozen specimens was analyzed by immunoblotting three times for each individual [26].
3D reconstructions were done from group III, using CT scans on fresh-frozen specimens.
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