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In order to examine formazan formation following MTT exposure, frozen tissues were embedded in Neg-50 frozen section medium and serial cross-sectioned.
The Fluorescein Fragel™ DNA Fragmentation Detection Kit (Calbiochem; VWR, Darmstadt, Germany,) was used according to the manufacturer's instructions in 5-μm-thick slices from the core of frozen tissues embedded in Neg-50 frozen section medium (Thermo Scientific, Richard-Allan Scientific, MI, USA).
In brief, brains were placed on frozen section medium Neg50 (Richard-Allan Scientific) for cryostat sections.
All tissue was sucrose protected and then frozen in a Neg50 frozen section medium (Richard-Allan Scientific, Kalamazoo, MI).
One day before sectioning, hearts were transferred into Neg-50™ frozen section medium (Richard-Allan Scientific, Kalamazoo, MI).
Excised tumors were embedded in frozen section medium (Richard-Allan Neg 50, Thermo Scientific, Waltham, MA), flash frozen in liquid nitrogen, and stored at -80°C until use.
Similar(43)
Conclusion: Digitized tissue smears are suitable for intraoperative neurotelepathology and provide comparable information to digitized frozen sections at medium power magnification.
Frozen section indicated the benign nature of the lesion.
Frozen section examination of the surgical stump showed no malignancy.
Pipelle and frozen section results were compared with final disease.
We did not undertake frozen section intraoperatively.
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