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All 17 hospitals had surgeons who could execute procedures requiring intraoperative frozen section analyses.
Intraoperative pathology methods currently being used for margin assessments include touch preparation cytology and frozen section analyses.
To our knowledge, our study will be the first to examine a regional telepathology project by focusing on intraoperative frozen section analyses.
These methods have limitations, however, including the requirement for an on-site trained pathologist, the inability to identify close margins (touch preparation cytology), and the ability to sample only a small portion of the margin (frozen section analyses) [ 12].
Pathologic touch preparation cytology and frozen section analyses are used in a select number of hospitals for intra-operative breast margin assessment and have been shown to have limitations, motivating the clinical need for improved intra-operative margin assessment tools [ 12].
With telepathology, intraoperative frozen section analyses can be performed whenever a pathologist is available at a remote location.
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For immunohistochemical analyses, both patellar and Achilles tissues were immersed in frozen section medium (Neg 50; Richard-Allan Scientific; Kalamazoo, MI, USA) in pre-labeled base molds and quickly frozen by placing in 2-methylbutane chilled in liquid nitrogen.
In our case, frozen section analysis was coherent with a GIST, but the final histological and immunohistochemical analyses demonstrated a leiomyosarcoma and excluded a sarcomatoid carcinoma and a GIST.
The resection plane of the specimen should be analysed histologically if possible already at the intraoperative fresh frozen section.
Pipelle and frozen section results were compared with final disease.
Frozen section indicated the benign nature of the lesion.
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