Sentence examples for frozen roots from inspiring English sources

Three hundred milligrams of frozen roots were crushed to a fine powder in a mortar under liquid nitrogen and homogenize with 3 ml of 25 mM potassium phosphate buffer (pH 7.8) containing 0.4 mM EDTA-4H, 1 mM ascorbic acid, and 2% PVPP.

Total RNA was extracted from frozen roots using TRIzol reagent (Invitrogen, Carlsbad, CA) following manufacturer's instructions.

Proteins were extracted from frozen roots and leaves using a phenol extraction procedure [ 16].

Total RNA was extracted from frozen roots with TRIzol (Invitrogen, Carlsbad, CA) according to the instructions from the manufacturer.

Proteins were extracted from frozen roots using a phenol extraction procedure according to Yang et al. [ 69].

Total RNA was isolated from frozen roots, seedlings, flower buds and leaves using the Trizol reagent according to manufacturer's instructions (Invitrogen, Carlsbad, CA).

Total RNA was extracted from 100 mg aliquots of frozen root material with innuPREP Plant RNA Kit (Analytik Jena, Germany) with an additional in-column DNAse digestion-step (RNase-Free DNAse Set, QIAGEN).

Briefly, frozen root tissues (2 g FW) were pulverized in a cold mortar with a pestle and then homogenized with ice-cold N2-purged 100 mM Tris HCl (pH 8.6), 1 mM phenylmethanesulfonyl fluoride (PMSF) and 1 % (v/v) Tween 20 at the ratio of 1 mL of buffer to 1 g tissue FW.

Frozen root tissues were stored at −80 °C until total RNA isolation.

Briefly, equal amounts of frozen root tips from six plants (one plant per pot) were mixed as a biological replicate.

Frozen root tissue was ground to a fine powder using mortar and pestle pre-chilled in liquid nitrogen.