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Resistance data stem from routine clinical testing (60% of tests) and from tests performed retrospectively from frozen repository plasma samples (40% of tests).
All of which suddenly made Oliver Ryder's frozen repository of skin samples a cellular gold mine.
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It's a pretty clunky, slow-loading (sometimes freezing), repository with a mere handful of available applications (listed here) – a far cry from what's available in the Android Market.
Given the rarity of well-annotated frozen tissue repositories, we sought to develop clinical outcome predictors using FFPE tissue.
The best performing GEMM-ESC clones are expanded and frozen as a repository for future experiments.
However, if there are importation issues (for example, if the source stock has a pathogen that is not allowed into your institutional animal facility), or if the mutant stock is frozen in a repository, then the CRISPR route may be more expedient and cost effective.
Blood was collected and serum prepared according to a standardised protocol within UKCTOCS (Menon et al, 2009) and then shipped frozen to a cryo-repository for long-term storage in liquid nitrogen.
Since 1998, the center has maintained a repository of plasma samples frozen at –70°C by retaining specimens obtained from patients during routine phlebotomy.
The elderberry fruits were harvested in 2010 from a germplasm repository in southwest Missouri United Statess) and frozen in zippered plastic freezer bags.
The samples were immediately flash frozen in liquid nitrogen and added to the repository of specimens stored at −80°C.
Aliquots of serum, plasma, and buffy coat were frozen and shipped on dry ice to a central repository and stored at −70°C for future assays in outside laboratories.
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