Sentence examples for frozen medium from inspiring English sources

Embryonic heads (E13.5 or younger), dissected brains (E14.5-P1 E14.5-P1nscardially perfused brains fror adultranscardiallye fixed in 4% formalin in perfusede-brainsed saline (PBS) at 4°C overnight, equilibrated in 30% sucrose and embedded in Tfromissue frozen medium (TBS) for frozen sectioning.

Cells were live-frozen in freezing medium (20% v/v DMSO; 80% v/v Fetal Calf Serum (Lonza, Basel, Switzerland)) in aliquots.

Monocytes were frozen for future use by suspending 10 × 106 cells/ml in ice cold freezing medium CTS® Synth-a-Freeze® Medium (Gibco).

Mixed stage animals grown in liquid CeMM can be directly mixed 1 1 with freezing medium [ 4, 7] and slowly frozen to -80°C.

The pancreatic tissue was processed for frozen histological analysis by embedding tissues in Tissue-Tek OCT freezing medium, and for formalin-fixed paraffin embedding (FFPE).

Lumbar spinal cords were embedded in OCT freezing medium and 10 mm-thick sections were prepared with a cryostat.

The biopsies were placed in freezing medium with 10% glycerol and frozen immediately at −20°C after the endoscopy, and moved to −70°C within 2 weeks.

Spinal cord tissue was embedded in OCT freezing medium, and 30 µm-cryostat sections were serially collected using a Leica cryostat.

For flow-cytometry analysis, these cells were either used directly or resuspended in freezing medium containing 10% DMSO and 90% FCS for storage in liquid nitrogen.

The temperature of freezing medium was 37°C.

Kidneys were rapidly excised and frozen in optimal cutting temperature freezing medium for preparation of cryosections.