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Frozen materials were microscopically examined for the determination of cancer cell content by pathologists, and it was confirmed that all 24 samples contained at least 40% cancer cells.
To confirm the specificity of the qPCR results, we characterized MCM2, MCM3, MCM7, as well as β-actin as a loading control, in six glioma samples for which freshly frozen materials were available.
Comparative studies of any other primate tissue have been limited to using post-mortem (typically frozen) materials, thereby precluding most experimental manipulation and yielding primarily observational insights (see, e.g., Blekhman et al., 2008; Blekhman et al. 2010; Brawand et al., 2011).
Total RNA was extracted from freshly frozen materials of lumbar spinal cords using the RNeasy Lipid Tissue Mini kit (Qiagen, Valencia, CA, USA), and in turn were used for RT to obtain cDNA using the Prime Script RT-PCR kit (Takara, Tokyo, Japan).
These results demonstrate that micro-CT and image analysis can be used to analyze the microstructure of frozen materials and obtain valuable information for designing the freezing process.
The detection of molecular minimal residual disease (MRD), however, requires fresh or frozen materials for designing clone-specific primers, which are not always readily available.
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Besides in-flight video observation, the experiments will be evaluated post-flight by means of fixed or frozen material.
Increasing air temperatures in the Arctic have the potential to degrade permafrost and promote the downward migration of the seasonally thawed active layer into previously frozen material.
The total flavonoids content was calculated using a calibration curve, expressed as mg quercetin/ml extract (R2 = 0.986) and then reported to 100 g frozen material.
The total polyphenols content was calculated using a calibration curve, expressed as mg gallic acid/ ml extract (R2 = 0.994) and then reported to 100 g frozen material.
The patient specific method considers cryopreservation as augmentation only among patients without a pregnancy from the fresh transfer, or from previously transferred frozen material from the same harvest.
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