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Besides in-flight video observation, the experiments will be evaluated post-flight by means of fixed or frozen material.
The total flavonoids content was calculated using a calibration curve, expressed as mg quercetin/ml extract (R2 = 0.986) and then reported to 100 g frozen material.
The total polyphenols content was calculated using a calibration curve, expressed as mg gallic acid/ ml extract (R2 = 0.994) and then reported to 100 g frozen material.
All tissue samples were collected as fresh frozen material.
Second, because the previous studies required frozen material, the number of specimens analyzed was limited.
The absence of contaminants was confirmed by plating frozen material on Chocolate agar.
DNA was extracted from the ground and frozen material with the DNAeasy kit (Qiagen, USA).
Another important aspect of this study was to evaluate the suitability of EXB Plus for comparison of FFPE tissue to fresh frozen material.
Frozen material was available from 352 out of 803 (44%) patients.
Frozen material was stored at −80° until RNA extractions were performed.
In this work, we have selected a set of intrahepatic cholangiocarcinomas, from frozen material.
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