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Frozen leaf material was milled in liquid nitrogen and extracted in 80% acetone.
Frozen leaf material was ground with 80%% ethanol at a ratio of 1 : 4 (w/v).
Total RNA was isolated from ground, frozen leaf tissues using the SpectrumTM Plant Total RNA Kit (Sigma-Aldirch).
Frozen leaf material from 8 accessions of Capsella grandiflora and 7 accessions of C. rubella was used in this study.
Frozen leaf material was pulverized in a mortar and an aliquot of 0.2 g plant powder placed in a glass vial with a septum in the lid.
For metabolite extraction, frozen leaf samples were ground into powders in liquid nitrogen with a mortar and pestle.
Briefly, 2 frozen leaf discs were extracted with 8 mL of 80% (v/v) acetone for 24 h in the dark.
The frozen leaf material was ground in a pre-cooled metal container with a 10 mm glass bead in a Braun Mikrodismembrator U for 90 sec at 2000 rpm.
Frozen leaf tissue was ground in a ball mill (Retsch, Haan, Germany).
Frozen leaf tissue was milled in a ball mill (Retsch, Haan, Germany).
Frozen leaf tissue was powdered with a mortar and pestle in liquid nitrogen.
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