Sentence examples for frozen in two from inspiring English sources

DNA and transformed lymphocytes were stored frozen in two different storage containers at two separate sites with continuous monitoring.

After 72 hours of incubation, supernatants were collected from the lymph node cell cultures and frozen in two aliquots at -80°C.

MNC derived from HUCB were frozen in three different solutions: (1) trehalose solution – 0.1 M trehalose in PBS (without Ca+2 & Mg+2).

Each center received peripheral blood mononuclear cells which were frozen in three different media.

Liver tissues were stored frozen in nine separate parts as a reservoir bank for subsequent RNA and genomic DNA analysis.

All participating laboratories received three vials of PBMCs from two donors, each frozen in three different media (A: serum, B: serum replacement, commercial, C: serum replacement, self-made).

A 10 20 g sample of mammary tissue was then surgically removed from each udder quarter in less than 10 min, snap frozen in three segments in liquid nitrogen and stored at -80°C.

While this sounds convenient, it will cause the cream to separate more than when it's frozen in one solid mass.

Exclusion criteria were (i) women with no supernumerary embryos for freezing in two previous treatment cycles, (ii) women ≥38 years of age with one previous cycle without freezing, (iii) couples using donor gametes and (iv) couples recruited to any other ongoing clinical trial.

We'd be freezing in four feet of snow and talking to friends down in Florida who were playing tennis and enjoying the beach.

However, postoperatively when 'OFF medication' during experiments, freezing in five patients was infrequent and then typically brief (<3 s).