And still they kept on coming, standing frozen in a long line on the stage to display their unquestioning loyalty to their anointed leader.
There is no nice way to put this, so I'm going to put it the way our three-year-old did when we showed him the old sepia-toned marriage photograph of two of his ancestors, their mouths frozen in that downturned line favoured by humans in those times, and by halibut in ours.
We analyzed 160 miRNAs expression levels in freshly fixed FFPE by comparing to snap frozen in a cell line model.
The first true leaf of each plant (21 days after sowing) was excised and pooled according to line and frozen in liquid nitrogen, giving a final harvest of four pooled leaf samples per line.
"The Ice Storm," (1997) for example, opens up with a shot of a Metro-North train on the New Haven line literally frozen in its tracks.
On the battlefields, an existential battle against genocidal terrorists is hampered by factionalism, with units failing to work together against the fanatical enemy, with the result that the front line has frozen in some places.
The HL-60 cell line was frozen in FCS supplemented with 10% dimethyl sulfoxide, and was kept in liquid nitrogen.
At the denoted time intervals, cotyledons were photographed and a minimum of 15 cotyledons per line was frozen in liquid nitrogen for chlorophyll extraction.
Similar to what was done for ASC2D/S-MSA and ASC7D-AD reprogramming, additional clones (at least 2+ per line) were frozen in bulk for potential future use.
The staging is full of familiar Wilson touches: the geometric backdrop illuminated with changing, saturated colors; minimal bits of scenery turned into landscapes; ensemble members crisscrossing the stage in straight intersecting lines; characters frozen in certain poses, and some stylized, angular hairdos.
