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When the plant is being frozen gradually, moisture leaves the cell to the outside of the membrane and forms ice crystals causing mechanical damage (Kim [2010]).
For collection of 20 µm cryostat sections (Bright), embryos were embedded in 1.5% Agarose/5% sucrose (Sigma), equilibrated in 30% sucrose solution overnight and frozen gradually on a metal tray cooled by contact with dry ice.
Cells were frozen gradually (in an isopropanol freezing container) to -80°C overnight and transferred afterwards to liquid nitrogen.
After performing a cell count, the cells were re-pelleted from Dulbecco's phosphate buffered saline solution and resuspended in Gibco Recovery Freezing medium (Invitrogen, Grand Island, NY), and then frozen gradually, prior to storage in liquid nitrogen.
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The silhouette of Ducky's toprock, footwork, and freezes gradually complement the glowing perpendicular lines until the momentum climaxes; and he basically jumps into this linear abyss.
During the ramp-up to such a condition, the latency to the onset of the activity burst during freezing gradually decreased.
But late last year, India began withdrawing its troops, and the freeze gradually began to thaw.
But water isn't the only substance that can freeze and gradually re-liquefy.
The fraction of flies freezing increased gradually with each looming presentation, arguing against habituation of looming stimulus detection (Fig. 2b).
The temperature was decreased to freezing temperatures gradually (2°C for 6 h, -2°C for 15 h, then 2°C decrease every 2 h).
Therefore, consumers attitudes toward frozen fish are gradually changing; it now appears that frozen fish is considered more desirable, but still not superior to its fresh/chilled counterpart, particularly in terms of its texture.
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CEO of Professional Science Editing for Scientists @ prosciediting.com