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On the same time we strongly suggest the further use of the same cell lines been transfected in this studies in Orthotopic Xenotransplantation Studies, as we best stable cells were frozen down already.
Aliquots were frozen down and thawed as needed for infections.
Correct clones were re-thawn, expanded and frozen down till their further use.
PBMCs were aliquoted and frozen down in liquid nitrogen for future use.
Mouse T cells were activated for 36 h, collected and frozen down at different time points post-infection.
Cells were then subjected to ficoll gradient purification and frozen down as described above for buffy coats.
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Nobody would find me, and I'd freeze down there.
"We froze down the stretch and didn't make plays," Weber said, visibly irritated.
Although the hut seemed warm enough, it was usually freezing down by the floor.
Cells were left undisturbed for 2 days, and split twice in a 1∶4 ratio before freezing down stocks.
We also froze down communities without fixing by directly embedding them in OCT (Piccirillo et al., 2010) and immediately freezing them on dry ice.
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