Sentence examples for frozen crushed from inspiring English sources
The cells and tissues were either frozen crushed in the rapid controlled rate freezer (NSF grant support to MM .or native disintegrated with ultrasonicator (Branson Ultrasonic, Danbury, CT, USA).
For selection and in vitro evolution, the HER-2 receptors were extracted from the human HER-2+ ovarian cancer cells by immunoprecipitation of rapidly frozen, crushed, thawed, and lyophilized.
The cells and tissues were either frozen crushed in the rapid controlled rate freezer (the NSF grant support to MM .or native disintegrated with ultrasonicator (Branson Ultrasonic, Danbury, CT, USA).
For selection and in vitro evolution, the HER-2 receptors were extracted from the human HER-2+ breast cancer cells by immunoprecipitation of rapidly frozen, crushed, thawed, and lyophilized.
Temperature was maintained by addition of frozen, crushed DMEM to the cell chambers.
Pericarp tissues from the five sampling points were immediately frozen, crushed in liquid nitrogen, and stored at −80 °C for RNA extraction, small RNA and degradome sequencing.
I was overjoyed when a vile missionary got his comeuppance; more than once, I suppressed a pang as yet another character I'd been sentimental enough to root for wound up frozen or crushed or tomahawked to death.
The expression of several cytokine genes was evaluated by real-time quantitative reserve transcription-PCR (RT-qPCR) in the brain of PbA-infected animals removed on day 7 p.i.Mice brains were frozen with crushed liquid nitrogen placed in the Trizol™ reagent (Invitrogen, USA) according to the protocol described by the manufacturer.
The tissues were submerged in liquid nitrogen, snap frozen and crushed using a mortar and pestle.
The brains were cryoprotected in 30% sucrose as described above, frozen in crushed CO2, and cut into sagittal or coronal, 18 μm thick, cryostat sections.
Four hours post-injection tumors were removed, frozen, and crushed into milligram-sized fragments, which were transferred individually to pre-weighed tubes containing RNAlater and then weighed.
