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Fig. 1 Schematic drawing Representing Bead Placement in the Medial Meniscus Fig. 2 Surgical Photograph and meniscal frozen cross section.
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For histopathologic analysis, the harvested quadriceps muscles were snap frozen in isopentene cooled in liquid nitrogen, and 10 µm frozen cross-sections were taken from the right quadriceps and subjected to hematoxylin/eosin (H&E), von Kossa (to highlight calcified fibers) and Masson's trichrome staining (to highlight areas of fibrosis).
Frozen tissue cross-sections were cut to a thickness of 10 μm in a cryostat at -20°C.
After allowing 6 hours for regeneration to be established, frozen 10 µm cross sections of whole animals were cut from anterior of the crush site to posterior of ganglion 9. Nine sections covering ganglion 9 from anterior (section 1) to posterior (section 9) were then imaged with MALDI-TOF in the region of the nervous system (Figure 1C, panel (2)).
The biopsies were cut in cross section and frozen in Tissue-Tek OCT compound (Electron Microscopy Sciences, Hatfield, PA). 12 μm thick cryostat sections were cut.
Frozen section cutting was aimed at obtaining a complete cross section at a single level and preventing tissue loss.
The cross section samples for the SEM imaging are commonly freeze fractured in liquid nitrogen.
After freezing the ultrasonographic image, the diameter of the veins was measured electronically in cross section from intima to intima of the vessel using the electronic cursors.
Finally, the cross section samples to the right were fractured at room temperature without freezing.
To carry out the characterization of a cross section of the PbS-PSNPs-SPEEK by SEM technique, nanocomposites samples were first frozen in liquid nitrogen for improving the breaking.
Cortical tissue was sliced in the cross section of barrel cortex at 20 µm by a freezing microtome.
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