Sentence examples for frozen cardiac from inspiring English sources

For detection of cardiac and glomerular macrophage infiltration, frozen cardiac and kidney sections were incubated overnight with the primary antibody rat anti-mouse CD68, Serotec; ×1000) followed by anti-rat secondary antibody (BioSource, Camarillo, CA, USA), as described previously [ 28].

For in vivo study, tissue sections (5 μm) from frozen cardiac tissues were fixed with 4% paraformaldehyde for 20 minutes and permeabilized in 0.1% Triton X-100 in 0.1% sodium citrate for 2 minutes at 4°C.

Frozen cardiac tissue was reduced to powder by means of a sterilized ceramic mortar and pestle.

As demonstrated in Fig.  6a and b, ROS generation in frozen cardiac tissue sections was examined.

Sections of frozen cardiac samples were assessed for collagen content by Trichrome staining as reported previously [ 12].

As shown in Figure  3a, ROS generation in frozen cardiac tissue sections in each group was examined in this study.

Behind her, the sharp thwock of the dense black rubber ball as it rockets from the paddle and slaps the wall, regular as a heartbeat till one of the men miscalculates and it freezes in cardiac arrest on the tail of a stifled curse.

To this end, we stained frozen sections of cardiac ventricular tissue as well as cardiac myocytes enzymatically isolated from mouse and rat hearts.

Frozen sections of cardiac muscle tissue were scanned as above, at 12 bit depth under non-saturating conditions.

Total RNA was prepared from snap-frozen cardiac tissues from 32-week-old male and female mice (n = 8 per diet group) using Qiagen RNeasy Kit (Quiagen, CA, USA) according to the manufacturer's instructions and stored at -80°C.

To obtain total protein extracts, AC16 cardiac cells or frozen tissue slides were lysed in cold RIPA buffer with phosphatase and protease inhibitors (0.2 mmol/l phenylmethylsulfonyl fluoride, 1 mmol/l sodium orthovanadate, 5.4 µg/ml aprotinin).