Sections of 30 µm thickness were cut on a cryostat microtome and, still frozen, applied onto the hydrophilic side of a 1.0×10.0 cm plastic support (GelBond, FMC, Rockland, ME, USA) (Figure 2B-1 and Figure 2B-2).
Cryogenic vials were kept on ice until samples were frozen applying the different freezing protocols (Table 1) using a rate controlled freezing system (Thermo Scientific) where indicated Model 7452 Series).
Slocum summarized that he "would experience the [butterfly] cut and then we would freeze, apply make-up [to create the cut on his skin], and then unfreeze and move on" filming.
The Venezuelan government insists that the freeze applies directly only to one American bank account (with $315m) belonging to PDVSA.
Yet the price freeze applies to several different brands of wine and liquor, six ice-cream desserts and 12 types of olives — as well as 22 deodorants.
The United States, which opposes all settlement construction, will not agree to that, an official said, although he said it was implicit that the 90-day freeze applies to the West Bank, not East Jerusalem.
This is not quite as dramatic as it first sounds: essential services will be maintained and pensioners will still get their Social Security checks as the freeze applies only to discretionary spending.
But this year's freeze applies to half the fiscal year, much longer than previous CRs.
"But the freeze applies to the entire budget, most of which doesn't relate to science.
The freeze applies to all match ticket prices, including season tickets and reduced-price Capital One Cup games.
