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Snap frozen ankles were prepared from mice 4 days after injection with K/BxN serum.
Frozen ankles joints in the carboxymethyl cellulose compound were attached to the adhesive Cryofilm (Finetec, Tokyo, Japan) and were cut in the microtome.
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A cadaveric fresh frozen ankle specimen (mid-tibia to include the foot) was used in the present study.
For example, she said, Sergeant Edmundson's hips, knees and ankles are frozen "in the position of someone sitting in a hallway in a chair".
"I was only in there for two minutes, but for some reason the socks froze to my ankles.
Joints such as elbows or ankles may become frozen in place; jaw motion can be impeded and the rib cage fixed, making eating or even breathing difficult.
Ankle joints were snap frozen in liquid nitrogen and homogenised in Qiagen RLT plus buffer (Qiagen, Crawley, UK) using Precellys24 ceramic bead homogenisation technology (Bertin Technologies, Montigny-le-Bretonneux, France).
Hind limbs, including the ankle joint were removed and snap frozen in liquid nitrogen.
After removal of the skin and muscle, portions of the leg containing the knee, ankle, and finger joints were rapidly frozen in liquid nitrogen.
The sleds bumped and dragged along frozen dirt berms, punishing the competitors to the tune of concussions, sprained ankles, busted knees, bruised hips, and dislocated fingers.
Ankle tissue (whole joints including synovium, bone and surrounding tissues) were excised post euthanasia and flash frozen in liquid nitrogen.
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