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Frontal tissue sections were cut at six to ten micrometers using a microtome and mounted on superfrost plus slide glasses (Microm France, Francheville).
Four patients with paired frontal tissue samples from separate sites according to intracranial electroencephalographic activities (cases 34 37, Table 1) showed no specific pathology, apart from mild inflammation.
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Grey matter was isolated from frozen postmortem frontal cortex tissue by dissection with a scalpel.
To test this hypothesis, we obtained postmortem human medial frontal gyrus tissue from BPD patients and healthy controls (n = 16).
A new device, the Utero-Spirotome (Fig. 1), offers the possibility of a direct and frontal (D&F) tissue harvest.
In another study, Rao et al. (2012) demonstrated increased methylation of BDNF promoter 1 in frontal cortex tissue in ten BD brains compared to ten age-matched controls (p < 0.05).
Application of this antibody termed Beclin-1 caspase-cleavage product antibody or BeclinCCP in frontal cortex tissue sections revealed strong immunolabeling within astrocytes that localized with plaque regions and along blood vessels in all AD cases examined.
Another postmortem brain study reported BDNF promoter hypermethylation in frontal cortex tissue in BD (Rao et al. 2012), in accord with the same group's previous finding of reduced BDNF mRNA in Brodmann area 9 of BD brains (Kim et al. 2010).
However, a representative sample of volumetric measurements of frontal connective tissue (white matter) has not been available.
Trypsin-dissociated primary cell cultures were prepared from frontal cortical tissue from 4-day-old (P4) Wistar rats (Charles River, Sulzfeld, Germany), using a previously described method [22], [29].
Immunohistochemistry using neurodegenerative process markers was not performed on frontal cortical tissue.
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