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We engaged the chamber to the front objective of optical tweezers, and adjusted the chamber axial position so that the laser focus falls inside the cell (Fig. 1(a)).
Fluorescence light from donor and acceptor dyes emitted from within a confocal volume was collected by the front objective, band-pass filtered, focused through a 20-µm pinhole, and imaged onto two avalanche photodiodes (APDs) (PerkinElmer, Waltham, MA).
If the distance to the target changes, you only refocus the front objective (or side focus).
Here is how to use equipment projection for binoculars: Cover the front objective lens of one side of the binoculars with a piece of card or a lens cap.
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"On a tactical front, our objective this quarter was to demonstrate a recovery from the challenging second quarter," James P. Gorman, the chairman and chief executive, said in a call with analysts.
However, in this case (i.e., when (varepsilon ) is not very small), we have a different type of difficulty: the width of the Pareto front on each objective in the normalized objective space is different [i.e., the nadir point in the normalized objective space is not (1, 1, (ldots ), 1)].
As a result, MOEA/D shows difficulties in finding a set of uniformly distributed solutions over the entire Pareto front when each objective has a totally different range of objective values.
A multi-objective genetic algorithm is used to determine the Pareto front for two objective functions: minimum wing drag and minimum wing weight.
LWD objectives enable the placement of an optional BK7 hemispherical lens in front of the objective lenses to further increase the NA.
White LEDs in front of the objective lens provide illumination for the CCD image.
Pictures were taken of the areas close to the sprout front with 20× objective for quantification of branch points.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com