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The results are interpreted in the context of neurological development, and, interestingly, these workers observe changes in elemental composition and in the sulfur XAS spectra of neuromelanin deposits with the age of the individual from which the brain sample was taken.
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Both models are plausible and although our subjects are from a longitudinal study, the brain samples are cross sectional which limits our ability to assess pathways and causality.
The brain samples were obtained from The Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam (open access: www.brainbank.nl).nl
Each age group had six rats, from which brain samples were taken, flash frozen, and stored at −80 °C.
The ΔCt from the reference brain samples was subtracted from the ΔCt obtained from the liver samples.
Within each brain region, expression profiles were highly correlated, irrespective of the hemisphere (left vs. right) and rat from which the sample was derived.
A selection of normal brain samples was obtained from BrainNet Europe/Barcelona Brain Bank and normal kidney samples were obtained from XBTC tissue bank.
Brain levels of GFAP were then determined in each individual from which the CSF samples were taken in the three AxD models.
The autopsied brain samples were obtained from Research Resource Network (RRN), Japan.
The homogenized brain samples were imaged under 60x magnification.
Three independent samples, each from two pooled adult brain samples were analyzed for each genotype.
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