The gene construct ECFP-SQS1 was then transferred from pUC vector to pC2300 vector and named pC ECFP-SQS1.
This is key to a deeper understanding of host, parasite and mosquito factors that participate in transmission of the parasite from host to vector and vector to host.
Finally, the third component (III), which relates to the percentage of successful infectious bites per infected host, includes the vector biting rates (a), the transmission rates from cattle to vector (b2) and the vector death rates.
Relative transcriptional activity was expressed as a ratio of luciferase reporter gene activity from the experimental vector to that from the internal control vector.
The pipelines creating targeted mutations proceed from gene annotation to vector design and vector construction, targeting in ES cells and microinjection or aggregation to produce mutant mice.
Supervised tensor learning can be formulated as the optimization problem of support tensor machines (STMs) [15] which is a generalization of the standard support vector machines (SVMs) from vector data to tensor data.
where e x is the unit vector along the x-axis of the global coordinate system, and ∡ stands for the function that maps the two vectors in the argument to the angle between them, where the angle is measured in the counterclockwise direction from the second vector to the first vector in the argument.
A recombination (LR) reaction is then performed which allows to transfer the cDNA from this entry vector to the destination vector (pBS-RfA).
The pooled plasmids were then converted from the pDONR221 vector to the pTP262 destination vector in batch using LR Clonase recombination enzyme [28].
The pooled plasmids were then converted from the pDONR vector to the pTP262 destination vector in batch using Clonase recombination enzyme in 10 µl reaction volume.
