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Extraction methods designed to optimize CNA recovery from touched articles resulted in comparable yields in a general population study, however the methods resulted in a twofold increase in DNA yields from touched items touched by individuals with sweaty hands.
The DNA profiles generated from touched objects are interpreted in respect to the person of interest's association with the criminal event and/or crime scene.
Low copy number DNA analysis offer the sensitivity to allow for analysis of minute amounts of DNA retrieved from touched objects.
Typical examples for the latter group are e.g. micro-stains, swabs from touched items, or samples that suffered from DNA degradation due to unfavourable environmental conditions and/or storage over prolonged time spans.
As depicted in Figure S3 this could be verified in principle by the successful typing of gDNA extracted from a test-panel of nonprobative forensically relevant samples comprising simulated single- and multi-contributor crime scene stains, such as hairs, cigarette butts, chewing gum, swabs from touched items, and old teeth, as well as reference samples (buccal swabs).
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The Pentagon memo, among other directives, barred military police from touching the Koran.
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